生长猪真可消化磷需要量的研究     

张永刚  李铁军  黄瑞林  印遇龙  张平  范明哲  方热军  汤海林  周绍儒 《中国科学院研究生院学报》2006,23(4):500-508

采用单因子随机分组设计,以真可消化磷(TdP)为指标,配制了5种生长猪试验日粮,并使其总钙与TdP的比例为2∶1.通过对生长性能和血清生化指标等的评定,对TdP需要量进行了初步研究.结果为:(1)得出生长猪平均日增重(ADG)随日粮TdP变化的多元方程;(2)拟合出了料肉比(F/G)随日粮TdP含量变化的多元方程;(3)根据血清无机磷浓度与日粮TdP含量的关系,也拟出了多元方程;(4)血清碱性磷酸酶(ALP)活性随着日粮TdP含量的升高呈下降趋势(P>0·05);(5)血清钙浓度基本不受日粮真可消化磷含量的影响.血清钙浓度随日粮钙含量的变化趋势,与血清无机磷随日粮TdP含量变化的趋势相似,且各组之间差异不显著(P>0·05).由此,本研究推荐生长猪真可消化磷的需要量为0·34%,相应地,总钙的需要量为0·68%.  相似文献   

人血清中微量铜的化学发光法测定   总被引：2，自引：0，他引：2  

吕小虎  陆明刚 《科技通报》1992,8(1):27-29

基于Cu~(2+)对鲁米诺-MnO_4~-化学发光反应的抑制作用建立了血清铜含量的化学发光分析法.测定铜(Ⅰ)的检出限为8.6×10~(-9)/ml,线性范围为1.0×10~(-8)～2.0×10~(-6)g/ml;对含1.0×10~(-7)g/ml cu~(+2)的水溶液进行10次重复测定,其结果的变异系数为4.3％.将本法应用于人血清中铜含量的测定,结果满意。  相似文献   

Serum gamma glutamyl transferase (γ-GT) activity in alcoholics with liver abscess and chronic alcoholics     

G. Rajagopal  K. M. Mohammed Rafi 《Indian journal of clinical biochemistry : IJCB》2005,20(2):198-199

Gamma Glutamyl Transferase (γ-GT) is a microsomal enzyme. Its activity in serum is assayed in alcoholics with liver abscess and alcoholics without liver abscess after screening by ultrasonography. The enzyme activity in serum is increased by 334% in the patients with liver abscess compared to normal control subjects (p<0.0001), and 172% when compared to patients with chronic alcoholism without liver abscess (p<0.0001). The enzyme activity was increased by 59.3% in the serum of patients without liver abscess compared to the normal control subjects (p<0.001). Measurement of γ-GT in serum appears to be a sensitive index in the diagnosis of alcoholics with liver abscess.  相似文献   

DETERMINATION　OF　CYCLOTRIME－THYLENETRINITRAMINE　IN　SERUM　OF　RABBIT　BY　RP－HPLC     

Chen Yong  Yuan Zhuo-bin 《中国科学院研究生院学报》1995,(2)

ＤＥＴＥＲＭＩＮＡＴＩＯＮＯＦＣＹＣＬＯＴＲＩＭＥ－ＴＨＹＬＥＮＥＴＲＩＮＩＴＲＡＭＩＮＥＩＮＳＥＲＵＭＯＦＲＡＢＢＩＴＢＹＲＰ－ＨＰＬＣＣｈｅｎＹｏｎｇ；ＹｕａｎＺｈｕｏ－ｂｉｎ（ＧｒａｄｕａｔｅＳｃｈｏｏｌ，ＡｃａｄｅｍｉａＳｉｎｉｃａ．Ｂｅｉｊ...  相似文献   

Blood zinc protoporphyrin, serum total protein, and total cholesterol levels in automobile workshop workers in relation to lead toxicity: Our experience     

Suneesh Kumar Pachathundikandi  Earaly Thomas Varghese 《Indian journal of clinical biochemistry : IJCB》2006,21(2):114-117

Blood zinc protoporphyrin (ZPP), serum total protein (TP), and total cholesterol (TC) levels in automobile workshop workers in relation to lead toxicity were analysed. In the present study, automobile workshop workers (healthy male workers at an age between 28 and 35 from four major automobile workshops in Kottayam, Kerala State, India) and the control (male healthy adults at an age between 28 and 35 residing at Aymanam, a distant village at Kottayam District, Kerala having reduced or no chance of lead exposure) displayed significant difference in blood lead (BPb) and blood ZZP (BZPP) level. The mean value of BPb in automobile workshop workers was 15.76±0.33 μg/dl, while in the control it was 8.20±0.15 μg/dl. In automobile workshop workers, the mean value of BZPP was 34.2±0.62 μg/dl. The control group exhibited a mean of 11.5±0.22 μg/dl. Automobile workshop workers exhibited significant increase in BZPP was corresponding to the increase in BPb level. The total protein levels estimated in automobile workshop workers showed significant decrease compared to control individuals, but was within the reference range of healthy individuals. The mean value of TP level in automobile workshop workers and control was 6.9±0.13 g/dl and 7.71±0.18 g/dl, respectively. There was no significant difference in blood haemoglobin (BHb) level among the automobile workshop workers and control. The serum TC level in automobile workshop workers showed significant decrease compared to the control individuals, but was with in the reference range of healthy individuals. The mean level of serum TC in automobile workshop workers was 162.00±3.44 mg/dl and the same in control was 172.86±4.32 mg/dl. The present study affirms occupational lead toxicity in automobile workshop workers and its effect on serum protein and cholesterol levels.  相似文献   

Musth in elephants     

A. Rajaram 《Resonance》2006,11(10):18-27

Animal behaviour is intimately connected with chemical signaling, often through glandular secretions. This is very significant and obvious in Asian male elephants during their musth period when their temporal glands secrete a fluid with a characteristic odor. Musth gland activity greatly influences the elephant society as well as manelephant interaction.  相似文献   

黄参多糖和游泳运动对营养性肥胖小鼠血脂及瘦素水平干预的实验研究   总被引：1，自引：0，他引：1  

贾磊  聂秀娟  肖雯  方梅 《西安体育学院学报》2011,28(6):710-714

目的通过黄参多糖(SGP)、游泳运动对采用高脂饮食建立的营养性肥胖小鼠血脂及瘦素水平进行干预,探索SGP及游泳运动的降脂减肥作用。方法本实验应用酶法测定了血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)﹑高密度脂蛋白(HDL-C)和应用酶联免疫法(ELISA)测定了小鼠血清瘦素,称取生殖器周围脂肪质量,计算肥胖指数(Lee’s index)和脂肪系数。结果高脂组(HFD)与基础对照组(Cont)比,体质量增加,Lee’指数和脂肪系数差异达到显著,证明通过高脂饮食建立营养性肥胖模型是成功的;应用SGP或游泳运动或二者联用均能降低营养性肥胖小鼠的体质量、体脂,降低血清中TC、TG及LDL-C,升高HDL-C水平,瘦素水平也随之在减肥中发挥作用而逐渐接近正常,其中以SGP和游泳运动联用产生协同作用效果最佳,与HFD组比差异达到极显著(P<0.01)。结论 SGP或游泳运动均能使营养性肥胖小鼠降脂、减肥,尤其是二者联用效果极为明显,为其在实践中应用提供了科学依据。  相似文献   

荧光光谱法研究黄豆苷原与牛血清白蛋白的相互作用     

宋胜梅  罗小芹  徐茂田 《商丘师范学院学报》2011,27(3):58-62

用荧光光谱法在pH7.4的PBS中研究了黄豆苷原（Daidzein,Da）与牛血清白蛋白（bovine serum albu-min,BSA）的相互作用.结果表明Da可静态猝灭BSA的内源荧光,温度升高时受动态猝灭的影响变得明显.二者之间的结合位点数约为1.5,315 K、310 K,300 K和290 K时的表观结合常数分别为5.44×104L.mol-1,5.70×104L.mol-1,5.29×104L.mol-1和5.69×104L.mol-1.焓变为0.2969 kJ.mol-1,熵变为91.78为J.mol-1.K-1,各温度下的自由能变均小于零,热力学参数表明二者之间主要靠静电力发生作用,熵变对反应过程具有较大贡献,且反应是自发过程.同步荧光光谱表明Da的加入改变了BSA的构像,使色氨酸残基的微环境亲水性增强.本研究对于阐明Da与蛋白的相互作用机理具有借鉴意义.  相似文献   

运动与限食对雄性肥胖大鼠血清睾酮的影响     

楼晓娟  李松波  张爱芳  汪毅 《北京体育大学学报》2011,34(7):48-50

目的:探讨运动与限食干预对青年雄性肥胖大鼠血清睾酮的影响。方法:采用双因素析因实验设计,肥胖模型建立后将肥胖大鼠按体重随机分为限食组、运动组、运动限食组、肥胖安静对照组。各组继续喂食高脂饲料,限食组喂食量为对照组喂食量的80%。游泳运动,5周,6 d/周,90 min/d。最后一次运动结束后60 min宰杀大鼠,分离血清测定睾酮。结果:1)对体重的影响:运动、限食主效应显著(P<0.001或0.01),交互作用不显著(P>0.05)。2)对大鼠血清睾酮的影响:运动与限食交互作用显著(P<0.01)。结论:青年雄性肥胖大鼠采用游泳运动方法减肥时,限食与否将对其血清睾酮产生不同的影响。  相似文献   

地美硝唑与牛血清白蛋白结合热力学特征的荧光光谱研究     

叶发兵  曾舟华  王伟 《黄冈师范学院学报》2008,28(6):12-16

采用荧光光谱和紫外可见吸收光谱法研究了地美硝唑与牛血清蛋白的结合反应,发现地美硝唑对牛血清白蛋白有较强的荧光猝灭作用,该猝灭过程主要为静态猝灭过程。从荧光光谱数据,由Stern—Volmer方程和Lineweaver—Burk方程分析并处理实验数据得到了23℃时结合反应的结合常数K=1．03×10^4L／mol,结合位点数为0．70,结合反应的标准焓变、标准熵变、和标准吉布斯自由能变分别为～63．48kJ／mol,-96．22J／K,-35．00kJ／mol。  相似文献